Although the program supports downloading of virus definitions through network connection but few network cards are supported. And it also does not support the USB 3.0 driver, if you boot it from USB plugged in USB 3.0, the program will not be able to find the required files on the USB drive. Windows Defender Offline is a WinPE 3.x version so the 32-bit version will not boot in UEFI. Windows Defender Offline is a lightweight Windows operating system, as a WinPE ( Windows Preinstallation Environment) version, which comes with Windows Defender built-in so you can use it to scan for viruses and spyware on your computer. Windows Defender Offline is a powerful offline scanning tool that runs from a trusted environment, without starting your operating system. See more information as well as download Windows Defender Offline ISO at this page. This article will guide you how to download Windows Defender Offline ISO and boot it from USB. Windows Defender Offline is a powerful offline scanning tool.